Occurrence of a single-species cyanobacterial bloom in a lake in Cyprus: monitoring and treatment with hydrogen peroxide-releasing granules

Environmental Sciences Europe

Table 1 Primers used in the detection of cyanotoxin producing genes in St. George Lake samples and amplification parameters of PCR

Targeting gene	Primer name	Sequence 5′–3’	Amplicon size (bp)	Annealing temperature (°C)	Reference or source of sequence for primers design
cyrJ	cyrJ_F	AGTAATCCCGCCTGTCATAGA	109	60	This study; KY550407.1
cyrJ	cyrJ_R	ACTGAGCATTGTCTCGGTAAAC	109	60	This study; KY550407.1
cyrB	cyrB_F	GCCTGAGTACCTATCTGCTTAAC	95	60	This study; EU140798.1
cyrB	cyrB_R	AGCCTGAAACTGCTCCATATC	95	60	This study; EU140798.1
sxtA	sxtA_F	GCGTACATCCAAGCTGGACTCG	683	55	[41]
sxtA	sxtA_R	GTAGTCCAGCTAAGGCACTTGC	683	55	[41]
anaC	anaC_F	TCTGGTATTCAGTCCCCTCTAT	366	58	[42]
anaC	anaC_R	CCCAATAGCCTGTCATCAA	366	58	[42]
mcyB	mcyB_F	CCTCAGACAATCAACGGTTAGT	119	60	This study; CP020771.1
mcyB	mcyB_R	AAAGGCAGAAGGCACCATATAA	119	60	This study; CP020771.1
mcyE	mcyE_F	CTGGTGGGAAAGGACTGATTTA	95	60	This study; CP020771.1
mcyE	mcyE_R	CGCCCTCAAGTCAAGAAAGA	95	60	This study; CP020771.1

The primers were designed using deposited sequences or taken from references. Cyr, sxt, ana and mcy genes are involved in cylindrospermopsin, saxitoxin, anatoxin and microcystin synthesis, respectively