Regrettable substitution? Comparative study of the effect profile of bisphenol A and eleven analogues in an in vitro test battery

Environmental Sciences Europe

Table 4 EC₅₀ values in the Microtox assay

Microtox	BPA	BPB	BPE	BPF	BPS	BPZ	BPAF	BPAP	BPBP	BADGE	TBBPA	TCBPA
EC₅₀ (mg/L)	13.2	12.2	19.4	8.50	39.1	8.50	5.81	8.36				16.2
95% confidence interval (mg/L)	12.8–13.6	12.0–12.5	19.0–19.9	8.27–8.68	37.2–41.1	8.26–8.74	4.37–7.73	6.73–10.4				15.0–17.4
Specific activity (M)	5.78 × 10⁻⁵	5.03 × 10⁻⁵	9.05 × 10⁻⁵	4.25 × 10⁻⁵	1.56 × 10⁻⁴	3.17 × 10⁻⁵	1.73 × 10⁻⁵	2.88 × 10⁻⁵				4.41 × 10⁻⁵
Maximum inhibition (%)	89.5	100	74.9	88.2	51.6	100	100	100	0.15	0.35	17.2	98.4
Significance to BPA	–	↓	↑	↓	↑	↓	↓	↓				↑

Displayed are the 50% effect concentrations (EC₅₀) (mg/L) with the respective 95% confidence interval (mg/L), specific activity (M), maximum luminescence inhibition (%), and the significant difference to BPA for each bisphenol. The specific activity is calculated using Eq. 1. The significant difference to BPA is determined through non-overlapping confidence intervals. Bold arrows pointing downwards indicate a significantly lower EC₅₀ than BPA, arrows pointing upwards indicate a significantly higher EC₅₀. Bisphenols with no values did not show an effect at the tested concentrations