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Environmental Sciences Europe

Table 5 Phytotoxicity test of crystal violet solutions before and after treatment by immobilized and un-immobilized spore laccase

From: Efficient immobilization of highly stable Bacillus amyloliquefaciens spore laccase for biodecolorization of textile dyes in water

Studied factor

Lentil seeds irrigated with

Water (Control)

Crystal violet (100 mg/L)

Dye solution decolorized by un-immobilized spore laccase

Dye solution decolorized by immobilized spore laccase

Germination (%)

90.0 ± 0.6 a

30.0 ± 0.6 d

70.0 ± 0.4 c

80.0 ± 0.7 b

Shoot length (cm)

7.8 ± 0.4 a

3.3 ± 0.1 d

5.7 ± 0.3 c

6.1 ± 0.3 b

Root length (cm)

2.3 ± 0.2 a

0.75 ± 0.1 d

1.7 ± 0.3 c

1.9 ± 0.1 b

Relative shoot length (%)

100 ± 0.0 a

42.3 ± 0.3 d

73.1 ± 0.5 c

78.2 ± 0.8 b

Relative root length (%)

100 ± 0.0 a

32.6 ± 0.7 d

73.9 ± 0.1 c

82.6 ± 0.6 b

Relative seed (%)

100 ± 0.0 a

33.3 ± 0.2 d

77.7 ± 0.4 c

88.8 ± 0.7 b

GI (%)

100 ± 0.0 a

10.8 ± 0.1 d

57.4 ± 0.4 c

73.3 ± 0.1 b

  1. Data were expressed as mean ± SE of 3 replicate tests. The means followed by different letters in each row are significantly different at P ≤ 0.05
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