Table 1 Primer sequence
Genes | Primer sequence | Product size (bp) | GenBank Accession number | Annealing Temperature (℃) |
|---|---|---|---|---|
IL-1α | F: CTG AAG GAG ATG CCT GAG ATA C | 383 | NM_000575.5 | 61 |
R: GAA CTG TCA ACA CTG CAC AAG | ||||
IL-1β | F: TAA AGA GAG CTG TAC CCA GAG A | 217 | NM_000576.3 | |
R: AAG TGA GTA GGA GAG GTG AGA G | ||||
TNF-α | F: CTC CTC ACC CAC ACC ATC AG | 134 | NM000594.4 | |
R: ATA GAT GGG CTC ATA CCA GGG | ||||
ITGαV | F: AAT CTT CCA ATT GAG GAT ATC AC | 140 | NM_002210.5 | |
R: AAA ACA GCC AGT AGC AAC AAT | ||||
LIF | F: CCA ACG TGA CGG ACT TCC C | 82 | NM000981.4 | |
R: TAC ACG ACT ATG CGG TAC AG | ||||
GAPDH | F: GGA GCG AGA TCC CTC CAA AA | 197 | NM_002046.7 | |
R: GGC TGT TGT CAT ACT TCT CA |