Fig. 5
From: Vitamin D receptor antagonist activity in wastewater effluents—potential for endocrine disruption
VDR antagonistic effects of NOM and cadmium, measured in VDR-UAS-bla HEK 293 T cells. Cells were treated with 0.05 nM 1α, 25-dihydroxyvitamin D3 before addition of 20–200 mg/L of NOM mixtures Nordic Aquatic Fulvic Acid mixture (NA-FA) and the Nordic Reservoir Natural Organic Matter (NR-NOM) (A) or 60 and 190 nM cadmium acetate (C). Cell viability was monitored by MTS test (B, D). Data is presented as mean ± standard deviation (n = 4)