Table 2 Comparison of cultivation conditions for CHO cell line one from this study and the study by Tan et al. [60]

Growth type

Suspension

Adherent

Medium

PowerCHO 2™ without fetal calf serum (protein free)

Ham’s F12 medium with 5% (v/v) fetal calf serum (protein containing)

Cultivation time after which CoCl₂ was added and subsequent treatment time with CoCl₂ [h]

0/up to 168 (Figs. 1, 2, 3)

24/ up to 144 (Additional file 1: Fig. S2)

24/16

Evaluation method

Determination of respiration activity

Determination of cellular clonal growth

Cytotoxicity (IC50) [mM] (cultivation time after addition of test substance [h] and subsequent treatment time [h] given in brackets)

0.111 (0/58)

0.030 (0/140–160)

0.036 (24/116–136)

0.140 (24/16)