Table 1 Overview of the combination of CHO cell lines, cultivation conditions, and test substances investigated within this study

Culture medium

PowerCHO 2 medium

sciNX medium

Initial viable cell concentration

0.2 ∙ 10⁶ cells mL⁻¹

0.5 ∙ 10⁶ cells mL⁻¹

IgG1 production?

Yes

No

Test substance

ZnCl₂

CoCl₂

CoCl₂

DMSO

Test substance concentrations

0.1 mM

0.01–0.3 mM

0.05–0.3 mM

0.1–5% (v/v)

Figure

1

Figures 1, 2, 3, Additional file 1: Fig. S1–3

Figure 4, Additional file 1: Fig. S4

Figures 5 + 6, Additional file 1: Fig. S5–7