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Environmental Sciences Europe
Fig. 3 | Environmental Sciences Europe

Fig. 3

From: Introducing oxygen transfer rate measurements as a novel method for time-resolved cytotoxicity assessment in shake flasks

Fig. 3

Dose–response curves for CoCl2 at different points in time using CHO cell line one. A Dose–response curve for CoCl2from cultivation data between 140 and 160 h. The following workflow was applied: OTR values were measured time resolved for different CoCl2 concentrations (cf. Fig. 2A). From each measured OTR value, cytotoxicity was calculated using Eq. 2 (cf. Fig. 2B). Calculated cytotoxicity values were averaged in the time interval between 140 and 160 h (cf. Fig. 2B). Obtained cytotoxicity values were plotted against the respective CoCl2 concentration to obtain the depicted dose–response curve. Logistic curve fitting according to Eq. 1 was performed to determine the IC50. IC50 = 30 µM. B Dose–response curve for CoCl2 after 58 h of cultivation. The same workflow as in A was applied, but single OTR values measured after 58 h were used. IC50 = 111 µM. Data from experiments 1 and 2 (Additional file 1: Table S1)

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