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Table 3 Coefficient of variation (CV) of cell numbers at the end of the test (72 h) for in vitro and in vivo measurements (absolute values as well as the ratio of the in vivo and in vitro values)

From: Testing particles using the algal growth inhibition test (OECD 201): the suitability of in vivo chlorophyll fluorescence measurements

Material Chlorophyll measurement Control 0.1 mg/L 1 mg/L 10 mg/L 1000 mg/L
Fibers
Cu CV in vitro 12.7 65.6 39.2 a a
CV in vivo 2.7 46.1 4.6 a a
Ratio in vitro/in vivo 4.7 1.4 8.5 a a
TiO2_fibers CV in vitro 6.4 18.3 34.8 1.2 3.3
CV in vivo 1.2 2.3 1.5 0.1 0.3
Ratio in vitro/in vivo 81.2 43.7 67.7 760.1 305.2
SiC_thin CV in vitro 13.8 14.0 33.5 55.8 32.3
CV in vivo 3.4 18.9 13.9 34.6 5.7
Ratio in vitro/in vivo 4.1 0.7 2.4 1.6 5.7
Platelets
Ag-ES-4 CV in vitro 18.1 17.8 20.0 16.1 0.0
CV in vivo 14.9 17.3 15.5 4.0 0.0
Ratio in vitro/in vivo 1.2 1.0 1.3 4.0 0.0
Spheres / compact particles
PMMA CV in vitro 36.6 n.d.b 15.4 7.0 31.2
CV in vivo 5.7 n.d.b 6.3 5.1 8.4
  Ratio in vitro/in vivo 6.4 a 2.4 1.4 3.7
PE_broad distribution CV in vitro 8.5 n.d.b n.d.b 17.7 11.5
CV in vivo 2.7 n.d.b n.d.b 5.3 3.9
Ratio in vitro/in vivo 3.1 a a 3.3 2.9
PA-6 CV in vitro 4.4 n.d.b n.d.b 12.1 10.2
CV in vivo 4.7 n.d.b n.d.b 2.5 9.1
Ratio in vitro/in vivo 0.9 a a 4.8 1.1
IN718 CV in vitro 30.3 51.1 3.2 42.2 33.8
CV in vivo 23.1 45.3 9.0 40.3 38.4
Ratio in vitro/in vivo 1.3 1.1 0.4 1.0 0.9
Ti64 CV in vitro 15.8 17.5 21.9 25.5 11.0
CV in vivo 5.3 17.8 13.2 18.2 6.9
Ratio in vitro/in vivo 3.0 1.0 1.7 1.4 1.6
IN-Y2O3 CV in vitro 18.4 n.d.b 6.9 10.2 7.0
CV in vivo 13.4 n.d.b 6.8 9.4 2.4
Ratio in vitro/in vivo 1.4 n.d.b 1.0 1.1 2.9
Ti64-SiC CV in vitro 10.3 36.8 22.8 9.7 4.5
CV in vivo 4.1 4.2 4.2 4.3 3.0
Ratio in vitro/in vivo 2.5 8.8 5.4 2.3 1.5
  1. a100% toxicity; no calculation possible. bn.d. = not determined