Fig. 4

Differential expressions of immune factors, transcription factors, and estrogen receptors in the spleen and their correlation with NP concentration. A RT-qPCR of ER-α and ER-β, NF-κB, NF-AT, AP-1, and differential immune cytokines (n = 6). B Immunoadsorption of ER-α, ER-β, NF-κB, NF-AT, AP-1 and IL-12 etc. C Protein quantitative analysis of ER-α, ER-β, NF-κB, NF-AT, AP-1 and IL-12 etc. (n = 6). D Correlation analysis of NP concentration in rat spleen tissues with protein expressions of ER-α, ER-β, NF-κB, NF-AT, AP-1 and IL-12 etc. Data were presented as the mean ± SEM, one-way ANOVA, ^avs. control group, P < 0.05; ^bvs. low NP group, P < 0.05; ^cvs. middle NP group, P < 0.05; ^dvs. high NP group, P < 0.05