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Fig. 6 | Environmental Sciences Europe

Fig. 6

From: Growth and chemotaxis of nematodes reduced upon exposure to Third Fork Creek surface water

Fig. 6

Nematode chemotaxis and growth decreased post-exposure to Third Fork Creek Surface Water collected in March 2020. C. elegans were exposed to surface water collected from Third Fork Creek on March 2 (A, B) and 9 (C, D), 2020 for 3 days. A To determine chemotaxis, nematodes present in the bacterial ring on each of the 3 days was recorded and the total number of nematodes in the bacterial ring on day three is presented in A. Significant differences were found when comparing Control and Sterile water (** denotes p = 0.0086) and Sterile Water and Third Fork Creek water sample (*** denotes p = 0.0010). No significant difference was found between Control and Third Fork Creek water sample (p = 0.1065). B To determine growth, live nematodes on each plate were counted and recorded for each of the 3 days and the total number of live nematodes on day three is presented in B. Significant differences were found when comparing Control and Sterile water (* denotes p = 0.0207) and Sterile Water and Third Fork Creek water sample (**p denotes p = 0.0024). No significant differences were found between Control and Third Fork Creek water sample (p = 0.1614). C To determine chemotaxis, nematodes present in the bacterial ring on each of the 3 days was recorded and the total number of nematodes in the bacterial ring on day three is presented in C. Significant differences were found when comparing Control and Third Fork Creek water sample and Sterile Water (*** denotes p = 0.0089), Control and Sterile water (** denotes p = 0.0002) and Sterile water and Third Fork Creek water sample (**** denotes p < 0.0001). D To determine growth, live nematodes on each plate were counted and recorded for each of the 3 days and the total number of live nematodes on day three is presented in D. Significant differences were found when comparing Control and Sterile water sample (*** denotes p = 0.0009), Control and Third Fork Creek water sample (* denotes p = 0.0125) and Sterile Water and Third Fork Creek water sample (**** denotes p < 0.0001). Significance was determined under a one-way ANOVA and Tukey’s post hoc test. Data are presented as the mean ± SEM for AD, n = 3

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