Fig. 4
From: Growth and chemotaxis of nematodes reduced upon exposure to Third Fork Creek surface water
No effect of TFC water samples on nematode chemotaxis and growth for summer sample collections. A C. elegans were exposed to surface water collected from Third Fork Creek on June 24, 2019 for 3 days. To determine chemotaxis, nematodes present in the bacterial ring on each of the 3 days was recorded and the total number of nematodes in the bacterial ring on day three is presented in A. No significant difference was found when comparing Control and Sterile water, Control and Third Fork Creek water sample and Sterile Water and Third Fork Creek water sample (p > 0.05). B C. elegans were exposed to surface water collected from Third Fork Creek on June 24, 2019 for 3 days. To determine growth, live nematodes on each plate were counted and recorded for each of the 3 days and the total number of live nematodes on day three is presented in B. No significant difference was found when comparing Control and Sterile water, Control and Third Fork Creek water sample and Sterile Water and Third Fork Creek water sample (p > 0.05). C C. elegans were exposed to surface water collected from Third Fork Creek on July 8, 2019 for 3 days. To determine chemotaxis, nematodes present in the bacterial ring on each of the 3 days was recorded and the total number of nematodes in the bacterial ring on day three is presented in C. No significant difference was found when comparing Control and Sterile water, Control and Third Fork Creek water sample and Sterile Water and Third Fork Creek water sample (p > 0.05). D C. elegans were exposed to surface water collected from Third Fork Creek on July 8, 2019 for 3 days. To determine growth, live nematodes on each plate were counted and recorded for each of the 3 days and the total number of live nematodes on day three is presented in D. No significant difference was found when comparing Control and Sterile water, Control and Third Fork Creek water sample and Sterile Water and Third Fork Creek water sample (p > 0.05). Significance was determined under a one-way ANOVA and Tukey’s post hoc test. Data are presented as the mean ± SEM for A–D, n = 3