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Table 3 Bioactivity observed in wetland water samples for the panel of bioassays used in the study

From: In vitro bioanalytical assessment of toxicity of wetland samples from Spanish Mediterranean coastline

  Cut-off
(fold-change)
EC/BEQ Wetland samples
1 2 3 4 5 6 7 8 9 10 11
AhR 2.0 EC10 24.7  > 25  > 25  > 25 19.5 10.6 13.3 7.9 21.6
TCDD-eq (pM) 10.2  < 7.7  < 6.9  < 9.3 12.4 20.5 17.1 22.2 11.3
AR AR+  1.5 EC50
DHT-eq (pM)
AR− 0.7 IC75 d
OHF-eq (pM)
ER ER+  1.5 EC50
E2–eq (pM)
ER− 0.8 IC75 d d d 3.21 3.84 d d
Ral-eq (nM) 441 23.1
Nrf2 1.5 ECIR1.5
tBHQ–eq (μM)
VDR VDR+  1.5 EC50
1,25-D3-eq (pM)
VDR− 0.7 IC75 d
  1. –: no activity detected above limit of detection at any REF, d: activity detected above limit of detection but no dose–response observed, EC10: sample REF value to produce 10% of the maximum effect produced by positive control, EC50: sample REF value to produce 50% of the maximum effect produced by positive control, IC75: sample REF value to produce 25% of the maximum antagonistic effect produced by positive control, ECIR1.5: sample REF value to induce a 1.5 fold-change activity versus vehicle control, BEQ: bioequivalent concentration, TCDD: 2,3,7,8-Tetrachlorodibenzodioxin, DHT: Dihydrotestosterone, OHF: Hydroxyflutamide, E2: 17β-estradiol, Ral: Raloxifene, tBHQ: Tert-butylhydroquinone, 1,25-D3: 1α, 25-dihydroxyvitamin D3