Skip to main content

Table 3 Bioactivity observed in wetland water samples for the panel of bioassays used in the study

From: In vitro bioanalytical assessment of toxicity of wetland samples from Spanish Mediterranean coastline

 

Cut-off

(fold-change)

EC/BEQ

Wetland samples

1

2

3

4

5

6

7

8

9

10

11

AhR

2.0

EC10

24.7

 > 25

 > 25

 > 25

19.5

10.6

13.3

7.9

21.6

–

–

TCDD-eq (pM)

10.2

 < 7.7

 < 6.9

 < 9.3

12.4

20.5

17.1

22.2

11.3

–

–

AR

AR+ 

1.5

EC50

–

–

–

–

–

–

–

–

–

–

–

DHT-eq (pM)

–

–

–

–

–

–

–

–

–

–

–

AR−

0.7

IC75

–

–

–

–

–

–

–

d

–

–

–

OHF-eq (pM)

–

–

–

–

–

–

–

–

–

–

–

ER

ER+ 

1.5

EC50

–

–

–

–

–

–

–

–

–

–

–

E2–eq (pM)

–

–

–

–

–

–

–

–

–

–

–

ER−

0.8

IC75

–

–

–

d

d

d

–

3.21

3.84

d

d

Ral-eq (nM)

–

–

–

–

–

–

–

441

23.1

–

–

Nrf2

1.5

ECIR1.5

–

–

–

–

–

–

–

–

–

–

–

tBHQ–eq (μM)

–

–

–

–

–

–

–

–

–

–

–

VDR

VDR+ 

1.5

EC50

–

–

–

–

–

–

–

–

–

–

–

1,25-D3-eq (pM)

–

–

–

–

–

–

–

–

–

–

–

VDR−

0.7

IC75

–

–

–

–

–

–

–

d

–

–

–

  1. –: no activity detected above limit of detection at any REF, d: activity detected above limit of detection but no dose–response observed, EC10: sample REF value to produce 10% of the maximum effect produced by positive control, EC50: sample REF value to produce 50% of the maximum effect produced by positive control, IC75: sample REF value to produce 25% of the maximum antagonistic effect produced by positive control, ECIR1.5: sample REF value to induce a 1.5 fold-change activity versus vehicle control, BEQ: bioequivalent concentration, TCDD: 2,3,7,8-Tetrachlorodibenzodioxin, DHT: Dihydrotestosterone, OHF: Hydroxyflutamide, E2: 17β-estradiol, Ral: Raloxifene, tBHQ: Tert-butylhydroquinone, 1,25-D3: 1α, 25-dihydroxyvitamin D3