Table 2 Panel of reporter gene assays for the assessment of toxicological fingerprint of wetland samples. Cell lines used, cytotoxicity assay method and positive control used for bioanalysis
Bioassay | Cell line | Cytotoxicity assay | Bioactivity positive control | |
|---|---|---|---|---|
Aryl hydrocarbon receptor (AhR) | Human hepatocellular carcinoma (HepG2) | MTS-based colorimetric assay | 2,3,7,8-Tetrachlorodibenzodioxin (TCDD) | |
Androgen receptor (AR) | Agonistic | Chinese Hamster Ovary (AR-EcoScreen) | MTS-based colorimetric assay | Dihydrotestosterone (DHT) |
Antagonistic | Hydroxyflutamide (OHF) | |||
Estrogen receptor (ER) | Agonistic | VM7Luc4E2 | CellTiter-Glo® Luminescent Cell Viability Assay | 17β-Estradiol (E2) |
Antagonistic | Raloxifene (Ral) | |||
Nuclear factor erythroid 2-related factor 2 (Nrf2) | MCF7C32ARE | MTS-based colorimetric assay | tert-Butylhydroquinone (tBHQ) | |
Vitamin D receptor (VDR) | Agonistic | VDR-UAS-bla HEK 293T | MTS-based colorimetric assay | 1α, 25-Dihydroxyvitamin D3 (1,25-D3) |
Antagonistic | n.a | |||