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Table 2 Panel of reporter gene assays for the assessment of toxicological fingerprint of wetland samples. Cell lines used, cytotoxicity assay method and positive control used for bioanalysis

From: In vitro bioanalytical assessment of toxicity of wetland samples from Spanish Mediterranean coastline

Bioassay Cell line Cytotoxicity assay Bioactivity positive control
Aryl hydrocarbon receptor (AhR) Human hepatocellular carcinoma (HepG2) MTS-based colorimetric assay 2,3,7,8-Tetrachlorodibenzodioxin (TCDD)
Androgen receptor (AR) Agonistic Chinese Hamster Ovary (AR-EcoScreen) MTS-based colorimetric assay Dihydrotestosterone (DHT)
Antagonistic Hydroxyflutamide (OHF)
Estrogen receptor (ER) Agonistic VM7Luc4E2 CellTiter-Glo® Luminescent Cell Viability Assay 17β-Estradiol (E2)
Antagonistic Raloxifene (Ral)
Nuclear factor erythroid 2-related factor 2 (Nrf2) MCF7C32ARE MTS-based colorimetric assay tert-Butylhydroquinone (tBHQ)
Vitamin D receptor (VDR) Agonistic VDR-UAS-bla HEK 293T MTS-based colorimetric assay 1α, 25-Dihydroxyvitamin D3 (1,25-D3)
Antagonistic n.a
  1. n.a. not available