Skip to main content

Table 1 Summary of RNA-Seq assembly results obtained for each genetically modified soybean variety under herbicide treatment

From: Stacked genetically modified soybean harboring herbicide resistance and insecticide rCry1Ac shows strong defense and redox homeostasis disturbance after glyphosate-based herbicide application

Sample Clean Reads > Q20 (%) GC (%) Total Mapped (%)
Control single-transgene variety (Replicate 1) 43,752,142 98.52 43.88 95.48
Control single-transgene variety (Replicate 2) 44,706,616 98.12 43.82 96.03
Control single-transgene variety (Replicate 3) 46,304,534 97.90 43.24 95.76
Treated single-transgene variety (Replicate 1) 49,058,110 98.46 43.90 96.11
Treated single-transgene variety (Replicate 2) 43,749,124 98.01 44.50 95.86
Treated single-transgene variety (Replicate 3) 42,710,012 97.91 44.77 94.01
Control stacked-transgene variety (Replicate 1) 52,509,694 98.50 43.58 96.33
Control stacked-transgene variety (Replicate 2) 44,592,750 98.06 43.07 96.58
Control stacked-transgene variety (Replicate 3) 40,277,576 97.96 43.50 96.00
Treated stacked-transgene variety (Replicate 1) 45,299,654 98.48 44.51 96.42
Treated stacked-transgene variety (Replicate 2) 42,526,766 97.89 44.49 95.92
Treated stacked-transgene variety (Replicate 3) 43,478,860 97.93 44.17 96.17
Average 44,913,820 98.14 43.95 95.89
  1. Single-transgene soybean variety (MON4032-6 event from Monsanto do Brasil S.A.) and stacked soybean variety (MON87701 and MON89788 from Monsanto do Brasil S.A.) were treated with glyphosate-based herbicide (Roundup Transorb® from Monsanto do Brasil S.A.) at 4.5 L/ha (2.2 kg a. i./ha) single dosage at V2 stage (treated samples). Leaf samples were collected for transcriptomic profiling 8 h after herbicide application. Control samples were not exposed to herbicide