Stacked genetically modified soybean harboring herbicide resistance and insecticide rCry1Ac shows strong defense and redox homeostasis disturbance after glyphosate-based herbicide application

Environmental Sciences Europe

Table 1 Summary of RNA-Seq assembly results obtained for each genetically modified soybean variety under herbicide treatment

Sample	Clean Reads	> Q20 (%)	GC (%)	Total Mapped (%)
Control single-transgene variety (Replicate 1)	43,752,142	98.52	43.88	95.48
Control single-transgene variety (Replicate 2)	44,706,616	98.12	43.82	96.03
Control single-transgene variety (Replicate 3)	46,304,534	97.90	43.24	95.76
Treated single-transgene variety (Replicate 1)	49,058,110	98.46	43.90	96.11
Treated single-transgene variety (Replicate 2)	43,749,124	98.01	44.50	95.86
Treated single-transgene variety (Replicate 3)	42,710,012	97.91	44.77	94.01
Control stacked-transgene variety (Replicate 1)	52,509,694	98.50	43.58	96.33
Control stacked-transgene variety (Replicate 2)	44,592,750	98.06	43.07	96.58
Control stacked-transgene variety (Replicate 3)	40,277,576	97.96	43.50	96.00
Treated stacked-transgene variety (Replicate 1)	45,299,654	98.48	44.51	96.42
Treated stacked-transgene variety (Replicate 2)	42,526,766	97.89	44.49	95.92
Treated stacked-transgene variety (Replicate 3)	43,478,860	97.93	44.17	96.17
Average	44,913,820	98.14	43.95	95.89

Single-transgene soybean variety (MON4032-6 event from Monsanto do Brasil S.A.) and stacked soybean variety (MON87701 and MON89788 from Monsanto do Brasil S.A.) were treated with glyphosate-based herbicide (Roundup Transorb^® from Monsanto do Brasil S.A.) at 4.5 L/ha (2.2 kg a. i./ha) single dosage at V2 stage (treated samples). Leaf samples were collected for transcriptomic profiling 8 h after herbicide application. Control samples were not exposed to herbicide