Fig. 2
Lithium suppresses the cell viability of human cardiomyocytes. a Microscopic images of AC16 cells treated with control (0 mmol/L) and LiCl at different concentrations (0.2 mmol/L, 1 mmol/L, 5 mmol/L or 25 mmol/L) for 48 h. Bars = 200 μm. b Influence of LiCl at different concentrations (0 mmol/L, 0.2 mmol/L, 1 mmol/L, 5 mmol/L or 25 mmol/L) on cell viability of AC16 cells as measured by luminescence assay 48 h post-cell seeding. The data are represented as mean ± SEM (n = 3). ***p < 0.001. c Influence of LiCl at different concentrations (0 mmol/L, 0.2 mmol/L, 1 mmol/L, 5 mmol/L or 25 mmol/L) on cell viability of AC16 cells as measured by CCK-8 assay 48 h post-cell seeding. The data are represented as mean ± SEM (n = 3). ***p < 0.001. d Microscopic images of AC16 cells treated with control (0 mmol/L) and NaCl at different concentrations (0.2 mmol/L, 1 mmol/L, 5 mmol/L or 25 mmol/L) for 48 h. Bars = 200 μm. e Influence of NaCl at different concentrations (0 mmol/L, 0.2 mmol/L, 1 mmol/L, 5 mmol/L or 25 mmol/L) on cell viability of AC16 cells as measured by luminescence assay 48 h post-cell seeding. The data are represented as mean ± SEM (n = 3). ***p < 0.001. f Influence of NaCl at different concentrations (0 mmol/L, 0.2 mmol/L, 1 mmol/L, 5 mmol/L or 25 mmol/L) on cell viability of AC16 cells as measured by CCK-8 assay 48 h post-cell seeding. The data are represented as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001. g Microscopic images of AC16 cells treated with control (0 mmol/L) and Li2SO4 at different concentrations (0.1 mmol/L, 0.5 mmol/L, 2.5 mmol/L or 12.5 mmol/L) for 48 h. Bars = 200 μm. h Influence of Li2SO4 at different concentrations (0 mmol/L, 0.1 mmol/L, 0.5 mmol/L, 2.5 mmol/L or 12.5 mmol/L) on cell viability of AC16 cells as measured by luminescence assay 48 h post-cell seeding. The data are represented as mean ± SEM (n = 3). ***p < 0.001. i Influence of Li2SO4 at different concentrations (0 mmol/L, 0.1 mmol/L, 0.5 mmol/L, 2.5 mmol/L or 12.5 mmol/L) on cell viability of AC16 cells as measured by CCK-8 assay 48 h post-cell seeding. The data are represented as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001