Combining morphological and metabarcoding approaches reveals the freshwater eukaryotic phytoplankton community

Environmental Sciences Europe

Table 1 Assigned or unassigned eukaryotic phytoplankton OTUs identified in 62 samples

	No. of assigned OTUs		No. of assigned reads		No. of unassigned OTUs		No. of unassigned reads		No. of total OTUs/reads
	SILVA	EPA	SILVA	EPA	SILVA	EPA	SILVA	EPA	No. of total OTUs/reads
Dictyochophyceae	9	21	5314	8368	15	3	5645	2591	24 (4.6%)/10,959 (0.5%)
Bacillariophyceae	37	53	372,783	328,519	18	2	5668	49,932	55 (10.5%)/378,451 (17.5%)
Dinophyceae	20	16	130,212	111,997	24	28	4560	22,775	44 (8.4%)/134,772 (6.2%)
Chrysophyceae	33	32	32,321	37,427	54	55	59,713	54,607	87 (16.6%)/92,034 (4.2%)
Cryptophyceae	40	38	377,469	296,149	7	9	31,291	112,611	47 (9.0%)/408,760 (18.9)
Eustigmatophyceae	11	11	49,346	49,429	6	6	5570	5487	17 (3.2%)/54,916 (2.5%)
Chlorophyta	100	158	302,888	631,754	122	64	720,919	392,053	222 (42.4%)/1023,807 (47.2%)
Euglenophyceae	3	3	97	97	0	0	0	0	3 (0.6%)/97 (0.0%)
Xanthophyceae	1	2	56	1452	5	4	7134	5738	6 (1.1%)/7190 (0.3%)
Charophyta	6	3	4958	801	2	5	1105	5262	8 (1.5%)/6063 (0.3%)
Haptophyta	2	3	13,510	13,527	1	0	17	0	3 (0.6%)/13,527 (0.6%)
Raphidophyceae	0	2	0	593	2	0	593	0	2 (0.4%)/593 (0.0%)
Bolidophyceae	1	1	1140	1140	0	0	0	0	1 (0.2%)/1140 (0.1%)
Leucocryptea	1	0	2266	0	1	2	33,030	35,296	2 (0.4%)/35,296 (1.6%)
Picophagophyceae	1	3	4	30	2	0	26	0	3 (0.6%)/30 (0.0%)
Sum	265 (50.6%)	346 (66.0%)	1,292,364 (59.6%)	1,481,283 (68.3%)	259 (49.4%)	178 (34.0%)	875,271 (40.4%)	686,352 (31.7%)	524/2,167,635

Assigned or unassigned OTUs defined as OTUs were assigned at genus and species levels or taxonomic ranks higher than genus when using RDP classifier against SILVA database and the evolutionary placement algorithm (EPA)