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Table 1 Characteristics of different behavioral tests

From: Hypo- or hyperactivity of zebrafish embryos provoked by neuroactive substances: a review on how experimental parameters impact the predictability of behavior changes

 

Spontaneous tail coiling test (STC)

Photomotor response test (PMR)

Locomotor response test (LMR)

Alternating light- and dark-induced locomotor response test (LMR-L/D)

Applied stimuli

Non

High-intensity light

Non

Alternating light/dark

Endpoint

Number or frequency of tail coilings

Movement activity or motion index

Swimming distance, duration, and speed

Swimming distance, duration, and speed

Exposure duration

0–28 hpf

0–42 hpf

0–120 hpf

0–120 hpf

Age of embryo at time of measurement

19–28 hpf

28–42 hpf

72–120 hpf

72–120 hpf