| Spontaneous tail coiling test (STC) | Photomotor response test (PMR) | Locomotor response test (LMR) | Alternating light- and dark-induced locomotor response test (LMR-L/D) |
---|---|---|---|---|
Applied stimuli | Non | High-intensity light | Non | Alternating light/dark |
Endpoint | Number or frequency of tail coilings | Movement activity or motion index | Swimming distance, duration, and speed | Swimming distance, duration, and speed |
Exposure duration | 0–28 hpf | 0–42 hpf | 0–120 hpf | 0–120 hpf |
Age of embryo at time of measurement | 19–28 hpf | 28–42 hpf | 72–120 hpf | 72–120 hpf |