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Table 3 The main methods used and expected outcomes of WP2: oil biodegradation and bioremediation

From: The EU Horizon 2020 project GRACE: integrated oil spill response actions and environmental effects

General experimental procedure Aims and expected outcome Refs.
Seawater microcosms with Crude oil water accommodated fraction (WAF) and addition of chemical dispersant at cold temperature. Chemical oil analysis and molecular biology analysis Oil and dispersed oil biodegradation rate and kinetic parameters at low temperature. Knowledge on main microbial taxa participating in oil biodegradation Reunamo et al. [2]
Venosa and Holder [44]
Sea ice experiments with encapsulated oil in laboratory scale. Chemical oil analysis and molecular biology analysis Natural degradation rate of crude oil in seasonal sea ice covered water. Knowledge on key microbial species and metabolic pathways responsible for biodegradation of oil Brakstad et al. [45]
Garneau et al. [46]
Omics data integration and meta-analysis of project-obtained and public domain data by recovery of individual genomes from obtained metagenomics datasets Information about microbial community taxonomic composition and metabolic markers. Better understanding of the role of uncultivated microbial species in oil biodegradation Huang et al. [47]
Klemetsen et al. [48]
Field pilot test with electrokinetic treatment of petroleum hydrocarbon contaminated marine sediment. Chemical oil analysis and molecular biology analysis of field samples Documented information on the performance of electrokinetic treatment as a method for marine sediments clean-up Masavat et al. [49]
Effect-based assessment of remediation success by cellular level bioassays Success of the remediation method evaluated for the mixed contamination using bioassays Brack et al. [50]