Table 3 The main methods used and expected outcomes of WP2: oil biodegradation and bioremediation
From: The EU Horizon 2020 project GRACE: integrated oil spill response actions and environmental effects
General experimental procedure | Aims and expected outcome | Refs. |
|---|---|---|
Seawater microcosms with Crude oil water accommodated fraction (WAF) and addition of chemical dispersant at cold temperature. Chemical oil analysis and molecular biology analysis | Oil and dispersed oil biodegradation rate and kinetic parameters at low temperature. Knowledge on main microbial taxa participating in oil biodegradation | Reunamo et al. [2] Venosa and Holder [44] |
Sea ice experiments with encapsulated oil in laboratory scale. Chemical oil analysis and molecular biology analysis | Natural degradation rate of crude oil in seasonal sea ice covered water. Knowledge on key microbial species and metabolic pathways responsible for biodegradation of oil | Brakstad et al. [45] Garneau et al. [46] |
Omics data integration and meta-analysis of project-obtained and public domain data by recovery of individual genomes from obtained metagenomics datasets | Information about microbial community taxonomic composition and metabolic markers. Better understanding of the role of uncultivated microbial species in oil biodegradation | Huang et al. [47] Klemetsen et al. [48] |
Field pilot test with electrokinetic treatment of petroleum hydrocarbon contaminated marine sediment. Chemical oil analysis and molecular biology analysis of field samples | Documented information on the performance of electrokinetic treatment as a method for marine sediments clean-up | Masavat et al. [49] |
Effect-based assessment of remediation success by cellular level bioassays | Success of the remediation method evaluated for the mixed contamination using bioassays | Brack et al. [50] |