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Environmental Sciences Europe
Fig. 1 | Environmental Sciences Europe

Fig. 1

From: Diuron modulates the DNA methylation status of the ILT7 and TRAIL/TNFSF10 genes and decreases the killing activity of plasmacytoid dendritic cells

Fig. 1

Schematic representation of the pDC exposure to Diuron and experimental schedule. a Molecular analyses (RT-qPCR, qMSRE, ELISA, ChIP) were performed 72 h after the Diuron exposure (25 mg/L). Lysis assay was performed 96 h after the Diuron exposure (25 mg/L). b The impact of Diuron on the viability of pDC was investigated using the XTT Cell Viability Kit (Cell Signaling, France). ABT737, a BH3 mimetic drug, was used as positive control for apoptosis induction. c Caspase-3 activity (Caspase-3 assay kit, Abcam, France) was measured to determine whether the pDC exposure to Diuron or ABT737 promoted apoptosis. Ctrl represents control exposure performed with DMSO

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