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Table 3 Test results with the reference substance azadirachtin

From: A new ecotoxicological test method for genetically modified plants and other stressors in soil with the black fungus gnat Bradysia impatiens (Diptera): current status of test development and dietary effects of azadirachtin on larval development and emergence rate

Test

Emergence rate

Development time

%MDD

CV in SC [%]

NOEC

LOEC

EC50 (95% CI)

CV in SC [%]

NOEC

LOEC

EC10 (95% CI)

RFT

5.2

25

125

160 (132–188)

2.7

25

125

6.19 (0–36.5)

10.5–11.2

CRT1

10.0

74

148

565 (413–717)

3.1

< 37

≤ 37

79.5 (78.8–238)

4.0–4.2

CRT2

22.5

< 37

≤ 37

88.6 (72.3–105)

4.8

< 37

≤ 37

18.7 (7.60–29.9)

5.7–6.1

  1. RFT range-finding test, CRT concentration–response test, CV coefficient of variation, SC solvent control, NOEC no-observed-effect concentration, LOEC lowest-observed-effect concentration, ECx X % effective concentration, CI confidence interval, MDD minimum detectable difference