Table 5 Observed developmental stages and endpoints during fish embryo test with Danio rerio; indicators of lethality are marked with an asterisk (*)

Mortality/coagulation*

X

X

X

X

X

X

X

X

Hatching

X

X

X

X

Developmental retardations

Epiboly

X

Gastrulation

X

Formation of somites*

X

Tail detachment*

X

Spontaneous movements

X

Eye development

X

Heart beat rate (beats/min)*

X

Otolith formation

X

Occurrence of melanocytes

X

Developmental failures

Edema (heart and yolk)

X

Malformation of eyes

X

X

X

X

Spinal deformation (scoliosis)

X

X

X

X

Pigmentation failures

X

X

X

X