Table 2 Sequences of primer pairs and corresponding amplicon sizes used in quantitative real-time PCR [11]
Transcript | Primer sequence (5′–3′) | Amplicon size (bp) |
|---|---|---|
Cytochrome P450 1a (cyp1a) | F: TTCGGAGCCGGTTTCGACAC R CCTCGAGGAGCGGCAGG | 166 |
ATP-binding cassette transporter C9 (abcc9) | F: CAGGGATGCACACGACATC R ACGGGAAGGACGGAAGAATG | 199 |
Pyruvate carboxylase (pc) | F: GTAAAGGTGAAGCCAGGCCAG R: TCCCCTTCCAGGCTGCTGTC | 146 |
Glycogen phosphorylase liver isoform (pygl) | F: TGGCCAATCACAGGATCGTTA R: TTCTCAATTGCCTCCACGTCA | 184 |
Protein kinase c delta type (pkcd) | F: TCCAGTAACAGCAACAGTTGAGA R: CCTGCTAGACATCTTGTTCATGC | 200 |
Extracellular superoxide dismutase (sod) | F: GAGTTCGACAACACAATCTATGCCAC R: CAGCCTTGACTGAGGTCTCC | 212 |
Transferrin variant d (tfd) | F: GGCACACTGGCAAGTTTACAT R: GGCTTTCAGGTGTCTTGCAG | 198 |
β-Actin—reference gene (actb) | F: CCGTAAGGACTTGTATGCCAACAC R: GGTGGGGCAATGATCTTGATC | 132 |