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Table 1 Standard methods for testing of anaerobic biodegradation (taken from[4], but up-dated)

From: A new method to determine the anaerobic degradability of surfactants: the AnBUSDiC test

  Standards Draft Standards Standards
  ASTM D 5210:1992(1) ASTM D 5511: 1994(2) DIN 38414, TL 8: 1985-06(3) ISO 11734: 1995 ISO/DIS 14853: 1999(4) ISO/DIS 15985: 1999(5) CEN/Draft: 1995(6) OECD 311: 2001(7) ECETOC: 1988(89)
Degradation parameter Biogas, CO2 and CH4, soluble organic carbon, residual polymer Biogas, CO2 and CH4 in gas phase Biogas in gas phase Biogas in gas phase and soluble inorganic carbon (IC) in liquid phase Biogas, CO2 and CH4, DOC, TIC resp. DIC Biogas, disintegration of test substance, optional CO2 and CH4 in gas phase Biogas and disintegration Biogas, DIC in liquid phase Biogas, DIC in liquid phase
Test substance polymer polymer   soluble organic substance Non-soluble (polymeric) substances Non-soluble (polymeric) substances packaging material div. Material div. Material
Medium definite mineral salt medium digested substance sewage sludge definite mineral salt medium definite mineral salt medium digested substance digested substance definite mineral salt medium definite mineral salt medium
Test volume 100 mL ca. 1 L in 2 L Erlenmeyer-flasks 500 mL 100 – 1000 mL 250 mL ca. 1000 mL ca. 1000 mL 100 – 1000 mL 100 – 1000 mL
Test duration   up to 70% degradation rete in reference substance 20 – 40 d 60 d 30 – 60 d 15 d (or longer) 15 d (or longer) 60 d 8 weeks
Temperature 35 ± 2°C 52 ± 2°C 35 ± 1°C 35 ± 2°C 35 ± 2°C 52 ± 2°C 52 ± 2°C 35 ± 2°C 35 ± 2°C
Method volumetric or manometric volumetric volumetric manometric volumetric or manometric volumetric (as example) Volumetric (as example) manometric manometric
Concentration test substance   15 – 100 g dry substance/L   100 mg/L organic carbon 100 mg/L organic carbon 20 g DS with 8 g TOC/L 20 g DS with 8 g TOC/L 20 – 100 mg/L organic carbon 20 – 50 mg/L organic carbon
Dry substance 1 – 2 g/L 300 g/L   1 – 3 g/L 1 – 3 g/L > 200 g/L > 200 g/L 1 – 3 g/L 1 – 5 g/L
  1. (1): Standard test method for determining the anaerobic biodegradation of plastic materials in the presence of municipal sewage sludge.
  2. (2): Standard test method for determining anaerobic biodegradation of plastic materials under high-solids anaerobic-digestion conditions.
  3. (3): Evaluation of digester behavior.
  4. (4): Evaluation of ultimate anaerobic biodegradation of plastic materials in an aqueous system – method by analysis of carbon conversion to carbon dioxide and methane.
  5. (5): Plastic – evaluation of the ultimate anaerobic biodegradability and disintegration under high-solids anaerobic-digestion conditions – method by analysis of released biogas.
  6. (6): Evaluation of ultimate biodegradation and disintegration of packaging materials under high-solids anaerobic digestion conditions – method by analysis of released biogas.
  7. (7): Ready anaerobic biodegradability; gas production from diluted anaerobic sewage sludge; OECD guidelines for testing of chemicals. Organization for Economic cooperation and development (2006).
  8. (8): ECETOC Technical Report No. 28: “Evaluation of anaerobic biodegradation; Guideline for screening of chemicals for anaerobic biodegradability” (ECETOC – European.
  9. Chemical Industry Ecology and Toxicology Centre).
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