Fig. 4From: Vitamin D receptor antagonist activity in wastewater effluents—potential for endocrine disruptionDose response of VDR antagonistic effects measured in VDR-UAS-bla HEK 293 T cells. Cells were treated with 0.05 nM 1α, 25-dihydroxyvitamin D3 before addition of the concentrated water samples, to activate the VDR signaling. Linear regression analysis was performed in GraphPad Prism 7. Data is presented as mean ± standard deviation (n = 4)Back to article page