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Environmental Sciences Europe

Table 3 Comet assay results of CHO cells exposed to maleic acid polymer scale inhibitor with 1/16 IC50, 1/4 IC50 and IC50 (0.084, 0.337 and 1.347 mg/mL, respectively)

From: General toxicity and genotoxicity studies of a new scale inhibitor for seawater desalination

Groups

Dose (mg/mL)

DNA in tail (%)

Tail moment

Maleic acid polymer scale inhibitor

   

 1/16 IC50

0.084

4.99 ± 0.37

2.35 ± 0.10**

 1/4 IC50

0.337

14.64 ± 1.14***

4.45 ± 0.39***

 IC50

1.347

25.11 ± 1.47***

9.14 ± 0.63***

Negative control

   

 Culture medium

–

5.27 ± 0.55

1.42 ± 0.16

Positive control

   

 K2Cr2O7

1 μg/mL

21.35 ± 1.01***

6.56 ± 0.33***

Typical Images. Typical examples of comet images of CHO cells in negative and positive control groups and treatment groups

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  1. The culture medium and K2Cr2O7 were negative and positive controls, respectively. Use a fluorescent microscope with a photographic system (eyepiece 10 × , Objective lens 20 × , Green light excitation absorption filter 590 nm) to obtain the images of comet cells, as shown in the "Typical Images". 100 random cells in each group were analyzed using Comet Assay IV™ software. All values are expressed as the mean ± SD. One-way ANOVA was used in the case of homoscedasticity, and the Brown–Forsythe test or Welch’s test was used in the case of heteroscedasticity for statistical analysis comparing with the control group. Significance was determined at p < 0.001*** and p < 0.01**
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