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Table 4 Potential outcomes of pre-tests to determine the suitability of in vivo fluorescence analysis and their interpretation

From: Testing particles using the algal growth inhibition test (OECD 201): the suitability of in vivo chlorophyll fluorescence measurements

Case

Suspension containing:

Interpretation

Suitability of in vivo fluorescence

Algae (i)

Materials (ii)

Algae + materials (iii)

1

Fluorescence signal

No/low fluorescence signal

Fluorescence signal comparable to (1)

No interference

Yes

2

Fluorescence signal

No / low fluorescence signal

Reduced fluorescence signal compared to (1)

Reduction of fluorescence by materials

No

3

Fluorescence signal

Fluorescence signal

Fluorescence signal

Autofluorescence of materials

No