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Environmental Sciences Europe
Fig. 3 | Environmental Sciences Europe

Fig. 3

From: Effects of subchronic exposure of nonylphenol on the expression of immune-related factors and estrogen receptors in the spleen of rats

Fig. 3

Changes of transcription factors, estrogen receptor ER-α, ER-β and IL-4 in rats after subchronic exposure to NP for 180 days (×200). A Changes of ER-α, IL-4, and ER-β in rat serum (n = 6). B Immunohistochemistry of ER-α and ER-β in the spleen (×200). C Immunohistochemical quantitative analysis of ER-α and ER-β protein expression in the spleen. D Immunofluorescence of NF-κB, NF-AT, and AP-1 in the spleen (×200). E Immunohistochemical quantitative analysis of NF-κB, NF-AT, AP-1 protein expression in the spleen (n = 9). Data were presented as the mean ± SEM, one-way ANOVA, avs. control group, P < 0.05; bvs. low NP group, P < 0.05; cvs. middle NP group, P < 0.05

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