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Fig. 5 | Environmental Sciences Europe

Fig. 5

From: Growth and chemotaxis of nematodes reduced upon exposure to Third Fork Creek surface water

Fig. 5

Nematode chemotaxis and growth decreased post-exposure to TFC Surface Water Collected in February 2020. C. elegans were exposed to surface water collected from Third Fork Creek on February 17 (A, B) and February 24 (C, D), 2020 for 3 days. A To determine chemotaxis, nematodes present in the bacterial ring on each of the 3 days was recorded and the total number of nematodes in the bacterial ring on day three is presented in A. No significant differences were found when comparing Control and Sterile water (p = 0.2025). Significant differences were found between Control and Third Fork Creek water sample (** denotes p = 0.0023) and Sterile Water and Third Fork Creek water sample (*** denotes p = 0.0005). B To determine growth, live nematodes on each plate were counted and recorded for each of the 3 days and the total number of live nematodes on day three is presented in B. Significant differences was found when comparing Control and Sterile water (** denotes p = 0.0013), Control and Third Fork Creek water sample (*** denotes p = 0.0004) and Sterile Water and Third Fork Creek water sample (**** denotes p < 0.0001). C To determine chemotaxis, nematodes present in the bacterial ring on each of the 3 days was recorded and the total number of nematodes in the bacterial ring on day three is presented in C. No significant difference found when comparing Control and Third Fork Creek water sample and Sterile Water. Significant differences were found when comparing Control and Sterile water (** denotes p = 0.0074) and Sterile Water and Third Fork Creek water sample (** denotes p = 0.0013). D To determine growth, live nematodes on each plate were counted and recorded for each of the 3 days and the total number of live nematodes on day three is presented in D. No significant difference was found when comparing Control and Third Fork Creek water sample. Significant differences between Control and Sterile water (** denotes p = 0.0060), Sterile Water and Third Fork Creek water sample (** denotes p = 0.0010). Significance was determined under a one-way ANOVA and Tukey’s post hoc test. Data are presented as the mean ± SEM for AD, n = 3

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