Table 2 Summary of the applied bioanalytical methods
Biological effect | Cellular endpoint | Cell line | Reference compound/positive control | LOD (fold change) | Cut-off (fold change) |
|---|---|---|---|---|---|
Adaptive stress response | Oxidative stress response (Nrf2 activity) | MCF7AREc32 | tBHQ (0.78–50 µM) | 1.31–1.40 | 1.5 |
Reactivity | Genotoxicity (micronuclei formation) | TK6 | Mitomycin C (100 nM) | – | * |
Xenobiotic metabolism | Aryl hydrocarbon receptor activation | DR-EcoScreen | TCDD (0.5–1000 pM) | 1.12–1.50 | 1.5 |
Modulation of hormone systems | Estrogen receptor agonism | VM7Luc4E2 | 17β-estradiol (0.358–367.1 pM) | 1.56–1.70 | 2.0 |
Androgen receptor agonism | AR-EcoScreen GR KO M1 | DHT (0.001–1000 nM) | 1.20–1.50 | 1.5 | |
Androgen receptor antagonism | AR-EcoScreen GR KO M1 | OHF (0.01–10,000 nM) | 0.76–0.88 | 0.70 |