Fig. 3

Behavioral response profiles of mean zebrafish swimming movement and speed in the dark (a) and in the light (b) each comprising 20 min (2 intervals of 10 min) after 96 h exposure to (±) anatoxin-a or caffeine. Behavioral parameters include swim distance, number of changes in movement (count), and swim duration in total and across 3 speeds, bursting (> 20 mm/s), cruising (5–20 mm/s), and freezing (< 5 mm/s). The tables below the graphs indicate a significant increase (↑) or decrease (↓) in activity compared to the negative control. In the (±) antx-a treatments, ANOVA and Dunnett’s post hoc were used to analyze treatment level responses compared to the negative control (*p < 0.10; **p < 0.05; ***p < 0.01). T tests were used to analyze caffeine influences compared to the negative control (^ p < 0.10; ^^ p < 0.05; ^^^ p < 0.01). A total of 24 zebrafish (4 replicates each with 6 larvae) were used for each treatment level