Table 1 Summary of effect-based methods used in this study
Endpoint | Cell line | Reference compound |
|---|---|---|
Estrogen receptor activation | Stably transfected T47D | 17β-Estradiol (E2) |
Androgen receptor activation | AR EcoScreen GR-KO M1 | Dihydrotestosterone (DHT) |
Androgen receptor antagonism | AR EcoScreen GR-KO M1 | Hydroxyflutamide (OHF) in addition to a fixed concentration of 200 pM DHT to stimulate the receptor |
Aryl hydrocarbon receptor activation | DR EcoScreen | 2,3,7,8-Tetrachlorodibenzodioxin (TCDD) |
Oxidative stress, Nrf2 activity | MCF-7/AREc32 | Tert-Butylhydroquinone (tBHQ) |