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Table 2 Exemplary assay working ranges for 2,3,7,8-TCDD (in pmol/L) matching QC criteria for calibrator imprecision and bias derived from various CALUX cell lines after fitting 5-PL functions by WSSR regression

From: Bioanalytical screening of low levels of dioxins and dioxin-like PCBs in pig meat (pork) for checking compliance with EU maximum and action levels using highly sensitive “third generation” recombinant H4L7.5c2 rat hepatoma cells

 

H4L7.5c2 rat (20 DREs)

H4L1.1c4 rat (4 DREs)

DR-CALUX rat (4 DREs)

H1L7.5c3 mouse (20 DREs)

H1L6.1c2 mouse (4 DREs)

RLU-based RSDa < 15%

0.1–300

0.3–300

0.3–300

0.1–300

0.3–300

Concentration-based RSDb < 15%

0.3–30

1.0–30

0.3–30

0.3–10

1.0–100

RLU-based biasc < 15%

0.3–300

0.3–30

0.3–30

0.3–30

1.0–100

Concentration-based biasc < 15%

0.3–30

0.3–30

1.0–30

0.3–30

1.0–100

Assay working range Ad

0.3–300

0.3–30

0.3–30

0.3–30

0.3–100

Assay working range Be

0.3–30

1.0–30

1.0–30

0.3–10

1.0–100

Sourcef

UCD

UCD

BDS

UCD

UCD

  1. aRequired according to EU legislation [18, 19]
  2. bRecommended according to EU legislation [18, 19]
  3. cSuggested by the authors
  4. dRange A: fulfilling RLU-based criteria for precision and bias
  5. eRange B: fulfilling RLU- and concentration-based criteria for precision and bias
  6. fUCD: University of California Davis, Davis (USA); BDS: BioDetection Systems, Amsterdam (The Netherlands)