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Fig. 2 | Environmental Sciences Europe

Fig. 2

From: Bioanalytical screening of low levels of dioxins and dioxin-like PCBs in pig meat (pork) for checking compliance with EU maximum and action levels using highly sensitive “third generation” recombinant H4L7.5c2 rat hepatoma cells

Fig. 2

a 2,3,7,8-TCDD concentration–response curve obtained from H4L7.5c2 rat hepatoma cells with a 5-parameter logistic (5-PL) equation fitted to concentration–response data pairs by weighted sum of squared residuals regression (WSSR). Calibration Pts corr.: calibration data corrected for the assay background response. Maximum response: 4730 RLUs, minimum response: 0 RLU, EC50: 11.4 pmol/L, EC70: 22.3 pmol/L, inflection point: 7.2 pmol/L, slope: 1.17, asymmetry factor: 1.51. b Calibrator bias: RLU-based bias ≤ 11% in range 0.3–300 pmol/L, conc.-based bias ≤ 6% in range 0.3–30 pmol/L. c Calibrator imprecision: RLU-based RSD ≤ 4% in range 0.1–300 pmol/L, conc.-based RSD ≤ 12% in range 0.3–30 pmol/L. RLU-based (solid blue line): based on relative light units, Conc.-based (dotted black line): based on back-fitted concentrations

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