Challenges in the regulatory use of water-accommodated fractions for assessing complex substances

Environmental Sciences Europe

Table 1 Acute and chronic aquatic toxicity data and calculations used for CTPHT classification by the summation method (RAC [29])

PAH	Measured mean concentration (ng/L) in WAF test at CTPHT loading rate of 100 mg/L	Acute classification							Chronic classification
PAH		Lowest aquatic E/LC50 (mg/L)	Content in CTPHT (% w/w)	Multiplying factor (M)	M x content in CTPHT (% w/w)	Concen tration limit (%)	Content in CTPHT/ concentration limit	Content in CTPHT (% w/w)/ E/LC50	Lowest aquatic NOEC or EC10 (mg/L)	M	M x content in CTPHT (% w/w)/ E/LC50
Naphthalene	< LOQ	1.0	0	1	0	25	0	0	0.020	1	0
Acenaphthylene	< LOQ	1.8	0		–	–	–	0	0.064	1	0
Acenaphthene	63.3	0.58	0.043	1	0.043	25	0.00172	0.074	0.038	1	0.043
Fluorene	53.9	0.025	0.047	10	0.47	2.5	0.0188	1.88	0.025	1	0.047
Anthracene	56	0.001	0.13	1000	130	0.025	5.2	130	0.0015	10	1.3
Phenanthrene	252	0.051	0.63	10	6.3	2.5	0.252	12.4	0.01	10	6.3
Pyrene	240	0.00023	0.95	1000	950	0.025	38	4130	0.0005	100	95
Chrysene	80.1	0.0007^a	0.81	1000	810	0.025	32.4	1157	ND	1000^b	810
Benz[a]anthracene	71.9	0.0018	0.77	100	77	0.25	3.08	428	0.0012	10	7.7
Fluoranthene	318	0.0001	1.1	10000	11000	0.0025	440	11000	0.0006	100	110
Benzo[a]pyrene	35.4	0.0012^a	1.0	100	100	0.25	4	833	0.0005	100	100
Benzo[b]fluoranthene	33.2	> WS	1.2	–	–	–	–	–	ND	NC	NC
Benzo[k]fluoranthene	< LOQ	0.00065	0.61	1000	610	0.025	24.4	938	0.00017	100	61
Dibenz[a,h]anthracene	< LOQ	0.0018	0.17	100	17	0.25	0.68	94.4	0.00014	100	17
Benzo[ghi]perylene	< LOQ	> WS	0.87	–	–	–	–	–	0.000082	1000	870
Indeno[1,2,3-cd]pyrene	< LOQ	0.00027	0.91	1000	910	0.025	36.4	3370	0.00027	100	91
Total	1204 (0.0012%)		9.2		14521		581	21280			2169

LOQ limit of quantitation, WS water solubility
^avalue derived in presence of UV light, ND no data; ^bbased on acute data; NC not classified