Fig. 2
Teratogenic effects of the AEO-7 surfactant on zebrafish embryos. a Representative photographs of 96-hpf embryos exposed to DEAB nanoparticles (positive control), PTU medium (negative control), or AEO-7. Note the edema in the yolk sac (yellow arrow) and heart (red arrow) after exposure to 10 µM DEAB. These images were captured with a Zeiss Stemi 2000-C stereomicroscope (×21). b The survival rate of embryos exposed to different concentrations of AEO-7 and the positive and negative controls. c Dose–response curves used to calculate the LC50 for DEAB and AEO-7. d Average body length, e size of the yolk, and f eye size following treatment, as captured using the HCImage software and analyzed with version 1.52a of the ImageJ software version. c One-way analysis of variance (ANOVA) followed by the Dunnett test was used to compare the groups, **p < 0.01 and ***p < 0.001, n = 20