Time point | Food level | Prochloraz | RMSE | Observedb | Predictedc | MDRd |
---|
(μg/L) | SAa | CA | EA | LC50 | LC50 | SAa | CA | EA |
---|
Day 21 | Low food | 0 | – | – | – | 0.098 | – | – | – | – |
1 | 6.0 | 8.3 | 5.6 | 0.048 | 0.079 | 1.7 | 3.2 | 0.93 |
32 | 4.2 | 17 | 10 | 0.0066 | 0.0023 | 0.34 | 25 | 6.8 |
100 | 5.8 | 25 | 18 | 0.0003 | 0.0008 | 2.8 | 660 | 150 |
High food | 0 | – | – | – | 0.49 | – | – | – | – |
1 | 6.8 | 3.1 | 3.1 | 0.55 | 0.59 | 0.77 | 0.94 | 0.94 |
32 | 8.4 | 17 | 16 | 0.19 | 0.21 | 1.4 | 2.8 | 2.6 |
100 | 4.9 | 14 | 13 | 0.20 | 0.22 | 1.0 | 2.7 | 2.6 |
Day 7 | Low food | 0 | – | – | – | 0.13 | – | – | – | – |
1 | 5.9 | 9.7 | 6.8 | 0.053 | 0.11 | 2.1 | 2.8 | 1.4 |
32 | 4.1 | 15 | 9.8 | 0.012 | 0.017 | 1.4 | 14 | 6.2 |
100 | 7.7 | 25 | 19 | 0.001 | 0.0032 | 3.2 | 240 | 75 |
High food | 0 | – | – | – | 0.53 | – | – | – | – |
1 | 5.6 | 4.4 | 4.4 | 0.65 | 0.49 | 0.80 | 0.82 | 0.82 |
32 | 8.0 | 14 | 14 | 0.26 | 0.33 | 1.2 | 2.1 | 2.0 |
100 | 7.4 | 13 | 13 | 0.25 | 0.19 | 0.71 | 2.2 | 2.1 |
- Predictions with different approaches for combining stressors: Stress-Addition according to the Multi-TOX approach (SA), effect-addition (EA) and concentration-addition (CA). Whole curve estimates of combined toxicant effect: root mean square error (RMSE). Point estimate (LC50) of combined toxicant effect: model deviation ratio (MDR)
- aSA (stress addition) calculated according to the Multi-TOX approach. For low food 81% SA, for high food 38% SA
- bObserved LC50 calculated with the mean survival of all experimental repetitions, fitted with ECx-SyS
- cPredicted LC50 based on the concentration–response relationships of single toxicants modeled with stress addition according to the Multi-TOX approach
- dMDR values are calculated by dividing the predicted LC50 of SA, CA, EA by the observed LC50. For values of CA > 2, interactions between stressors are interpreted as synergistic related to CA