Table 1 Information regarding the main aspects addressed in OECD guidelines, where “NO CHANGE”, “CHANGE” or “TO DEVELOP”, “TO STANDARDIZE” (partly developed but needs standardization) are recommended for the hazard assessment of NBMs
Subjects addressed in the OECD environmental in vivo hazard protocols | |
|---|---|
Principle of the test | No change |
Test acceptance/validity criteria | No change |
Information on test substance | Change |
The need for reference test substances | To develop |
Reference substance—ORGANISMS | No change |
Reference substance—MATERIALS | To develop |
Description of the test | |
The need for adaptations to test NBMs | To standardize |
The need of instruments and techniques for environmental and biological media characterization | To develop |
Preparation of media | No change |
Selection and preparation of test animals | (No)change |
Preparation of test concentrations | Change |
Mixing the test substance to the media—the need for spiking and dispersion consensus | Change |
Performance of the tests | |
The need of testable materials and worst-case scenarios | To standardize |
Test groups and control | No change |
Test conditions and feeding | No change |
Test design—The need to cover whole material life cycle and The need of relevant test duration | Change |
Data and reporting | Change |
Treatment of results | Change |