Fig. 3

Lithium suppresses the cell proliferation of human cardiomyocytes. a EdU detection in AC16 cells treated with control and 5 mmol/L LiCl for 48 h. Nuclei were counterstained with DAPI. Bars = 200 μm. b EdU detection in AC16 cells treated with control and 2.5 mmol/L Li₂SO₄ for 48 h. Nuclei were counterstained with DAPI. Bars = 200 μm. c Cell number analyses of AC16 cells treated with control and 5 mmol/L LiCl for 48 h. The data are represented as mean ± SEM (n = 3). **p < 0.01, ***p < 0.001. d Cell number analyses of AC16 cells treated with control and 2.5 mmol/L Li₂SO₄ for 48 h. The data are represented as mean ± SEM (n = 3). **p < 0.01, ***p < 0.001. e, f Western blot analyses of the protein levels of PCNA in cells treated with control, 5 mmol/L LiCl and 2.5 mmol/L Li₂SO₄ for 48 h. The data are represented as mean ± SEM (n = 3). ***p < 0.001