Fig. 3
a Control status of liver of juvenile trout with homogenous tissue with large hepatocytes. b The hepatocytes contain a huge amount of glycogen. c Occurring reactions in the liver were inter-alia inflammations (IN), vascular dilation (VD) and focal necrosis (FN). d Furthermore, reduced glycogen amount and increased intercellular (IC) spaces in liver samples were found. e Control status of gills of juvenile trout with regularly shaped secondary lamellae. f Reaction status of gills showing hypertrophy (HT) and hyperplasia (HP) of cells, lamellar fusion (LF) as well as edema (OE). a, c, e, f Hematoxylin–eosin staining; b, d Alcian blue-PAS staining. g, h Semi-quantitative analyses of liver (g) and gills (h). Category 1 represents an excellent health status, 3 a reaction status and 5 a destruction status. 2 and 4 are intermediary classes. No section was assigned to category 5. Statistical comparison showed significantly more reactions in liver and gill samples of methiocarb or methiocarb plus PS-MP exposed fish. Different letters indicate significant differences (p < 0.0001)