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Table 3 Summary of test conditions used for transformation studies to investigate the influence of type and origin of manure on transformation rates (Test series 2)

From: Development of a test method for transformation of veterinary pharmaceuticals and biocides in anaerobic liquid manure

Parameter

Influence of type and origin of manure on transformation rates (Test series 2)

Test setup

Flow-through: slow flow (50–200 mL/min)

Flow-through: slow flow (50–200 mL/min)

Flow-through: slow flow (50–200 mL/min)

Test substance

14C-salicylic acid

14C-paracetamol

14C-biocide B

Temperature

20 ± 2 °C

20 ± 2 °C

20 ± 2 °C

Light

Darkness

Darkness

Darkness

Acclimation period

21 days

21 days

21 days

Type of manure

Cattle (c), pig (p)

Cattle (c), pig (p)

Cattle (c), pig (p)

Manure sampling

3 sites

c: winter + summer

p: winter/summer

3 sites

c: summer

p: winter/summer

3 sites

c: summer

p: summer

Dry matter content

10 ± 1% (c), 5 ± 1% (p)

10 ± 1% (c), 5 ± 1% (p)

10 ± 1% (c), 5 ± 1% (p)

Amount of manure

50 g fw

50 g fw

50 g fw

Replicates per sampling

6 (set in series)

6 (set in series)

6 (set in series)

Test duration

36 days (c, p)

28 days (c), 41 days (p)

105 days (c, p)

Sampling time points

c, p: 0, 3, 7, 14, 21, 28, 36

c: 0, 0.3, 1, 3, 7, 14, 28;

p: 0, 1, 3, 7, 14, 28, 41

c, p: 0, 7, 21, 42, 63, 84, 105

14CO2/14CH4 quantification

Yes (each sampling point)

Yes (each sampling point)

Yes (each sampling point)

Residue characterization

Yes (each sampling point)

Yes (each sampling point)

Yes (each sampling point)

Abiotic/sterile controls

Yes (sampling at the end)

Yes (sampling at the end)

Yes (sampling at the end)

Microbial activity testing

Yes (at start and end)

Yes (at start and end)

Yes (at start and end)

Complete mass balance

Yes (each sampling point)

Yes (each sampling point)

Yes (each sampling point)

Evaluation of kinetics

Yes (dissipation rate of parent, DT50, DT90)

Yes (dissipation rate of parent, DT50, DT90)

Yes (dissipation rate of parent, DT50, DT90)