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Table 3 Summary of test conditions used for transformation studies to investigate the influence of type and origin of manure on transformation rates (Test series 2)

From: Development of a test method for transformation of veterinary pharmaceuticals and biocides in anaerobic liquid manure

ParameterInfluence of type and origin of manure on transformation rates (Test series 2)
Test setupFlow-through: slow flow (50–200 mL/min)Flow-through: slow flow (50–200 mL/min)Flow-through: slow flow (50–200 mL/min)
Test substance14C-salicylic acid14C-paracetamol14C-biocide B
Temperature20 ± 2 °C20 ± 2 °C20 ± 2 °C
LightDarknessDarknessDarkness
Acclimation period21 days21 days21 days
Type of manureCattle (c), pig (p)Cattle (c), pig (p)Cattle (c), pig (p)
Manure sampling3 sites
c: winter + summer
p: winter/summer
3 sites
c: summer
p: winter/summer
3 sites
c: summer
p: summer
Dry matter content10 ± 1% (c), 5 ± 1% (p)10 ± 1% (c), 5 ± 1% (p)10 ± 1% (c), 5 ± 1% (p)
Amount of manure50 g fw50 g fw50 g fw
Replicates per sampling6 (set in series)6 (set in series)6 (set in series)
Test duration36 days (c, p)28 days (c), 41 days (p)105 days (c, p)
Sampling time pointsc, p: 0, 3, 7, 14, 21, 28, 36c: 0, 0.3, 1, 3, 7, 14, 28;
p: 0, 1, 3, 7, 14, 28, 41
c, p: 0, 7, 21, 42, 63, 84, 105
14CO2/14CH4 quantificationYes (each sampling point)Yes (each sampling point)Yes (each sampling point)
Residue characterizationYes (each sampling point)Yes (each sampling point)Yes (each sampling point)
Abiotic/sterile controlsYes (sampling at the end)Yes (sampling at the end)Yes (sampling at the end)
Microbial activity testingYes (at start and end)Yes (at start and end)Yes (at start and end)
Complete mass balanceYes (each sampling point)Yes (each sampling point)Yes (each sampling point)
Evaluation of kineticsYes (dissipation rate of parent, DT50, DT90)Yes (dissipation rate of parent, DT50, DT90)Yes (dissipation rate of parent, DT50, DT90)