Parameter | Influence of type and origin of manure on transformation rates (Test series 2) | ||
---|---|---|---|
Test setup | Flow-through: slow flow (50–200 mL/min) | Flow-through: slow flow (50–200 mL/min) | Flow-through: slow flow (50–200 mL/min) |
Test substance | 14C-salicylic acid | 14C-paracetamol | 14C-biocide B |
Temperature | 20 ± 2 °C | 20 ± 2 °C | 20 ± 2 °C |
Light | Darkness | Darkness | Darkness |
Acclimation period | 21 days | 21 days | 21 days |
Type of manure | Cattle (c), pig (p) | Cattle (c), pig (p) | Cattle (c), pig (p) |
Manure sampling | 3 sites c: winter + summer p: winter/summer | 3 sites c: summer p: winter/summer | 3 sites c: summer p: summer |
Dry matter content | 10 ± 1% (c), 5 ± 1% (p) | 10 ± 1% (c), 5 ± 1% (p) | 10 ± 1% (c), 5 ± 1% (p) |
Amount of manure | 50 g fw | 50 g fw | 50 g fw |
Replicates per sampling | 6 (set in series) | 6 (set in series) | 6 (set in series) |
Test duration | 36 days (c, p) | 28 days (c), 41 days (p) | 105 days (c, p) |
Sampling time points | c, p: 0, 3, 7, 14, 21, 28, 36 | c: 0, 0.3, 1, 3, 7, 14, 28; p: 0, 1, 3, 7, 14, 28, 41 | c, p: 0, 7, 21, 42, 63, 84, 105 |
14CO2/14CH4 quantification | Yes (each sampling point) | Yes (each sampling point) | Yes (each sampling point) |
Residue characterization | Yes (each sampling point) | Yes (each sampling point) | Yes (each sampling point) |
Abiotic/sterile controls | Yes (sampling at the end) | Yes (sampling at the end) | Yes (sampling at the end) |
Microbial activity testing | Yes (at start and end) | Yes (at start and end) | Yes (at start and end) |
Complete mass balance | Yes (each sampling point) | Yes (each sampling point) | Yes (each sampling point) |
Evaluation of kinetics | Yes (dissipation rate of parent, DT50, DT90) | Yes (dissipation rate of parent, DT50, DT90) | Yes (dissipation rate of parent, DT50, DT90) |