Test setup | Flow-through: slow flow (50–200 mL/min) | Flow-through: slow flow (50–200 mL/min) | Flow-through: slow flow (50–200 mL/min) |

Test substance | ^{14}C-salicylic acid | ^{14}C-paracetamol | ^{14}C-biocide B |

Temperature | 20 ± 2 °C | 20 ± 2 °C | 20 ± 2 °C |

Light | Darkness | Darkness | Darkness |

Acclimation period | 21 days | 21 days | 21 days |

Type of manure | Cattle (c), pig (p) | Cattle (c), pig (p) | Cattle (c), pig (p) |

Manure sampling | 3 sites
*c*: winter + summer
*p*: winter/summer | 3 sites
*c*: summer
*p*: winter/summer | 3 sites
*c*: summer
*p*: summer |

Dry matter content | 10 ± 1% (c), 5 ± 1% (p) | 10 ± 1% (c), 5 ± 1% (p) | 10 ± 1% (c), 5 ± 1% (p) |

Amount of manure | 50 g fw | 50 g fw | 50 g fw |

Replicates per sampling | 6 (set in series) | 6 (set in series) | 6 (set in series) |

Test duration | 36 days (*c*, *p*) | 28 days (c), 41 days (p) | 105 days (*c*, *p*) |

Sampling time points | *c*, *p*: 0, 3, 7, 14, 21, 28, 36 | *c*: 0, 0.3, 1, 3, 7, 14, 28;
*p*: 0, 1, 3, 7, 14, 28, 41 | *c*, *p*: 0, 7, 21, 42, 63, 84, 105 |

^{14}CO_{2}/^{14}CH_{4} quantification | Yes (each sampling point) | Yes (each sampling point) | Yes (each sampling point) |

Residue characterization | Yes (each sampling point) | Yes (each sampling point) | Yes (each sampling point) |

Abiotic/sterile controls | Yes (sampling at the end) | Yes (sampling at the end) | Yes (sampling at the end) |

Microbial activity testing | Yes (at start and end) | Yes (at start and end) | Yes (at start and end) |

Complete mass balance | Yes (each sampling point) | Yes (each sampling point) | Yes (each sampling point) |

Evaluation of kinetics | Yes (dissipation rate of parent, DT_{50}, DT_{90}) | Yes (dissipation rate of parent, DT_{50}, DT_{90}) | Yes (dissipation rate of parent, DT_{50}, DT_{90}) |