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Table 2 Summary of test conditions used for transformation studies to investigate the influence of test setup on mineralization rates of 14C-salicylic acid in cattle and pig manure (Test series 1)

From: Development of a test method for transformation of veterinary pharmaceuticals and biocides in anaerobic liquid manure

ParameterInfluence of test setup on mineralization rates (Test series 1)
Variations in test setup(i) Flow-through vs. semi-static(ii) Slow flow vs. fast flow(iii) Low vs. high amount of manure
DetailsFlow-through: 50–200 mL min−1
Semi-static: purged once/week
Slow flow: 50–200 mL/min
Fast flow: > 200 mL min−1
Low: 50 g manure fw
High: 300 g manure fw (slow flow-through)
Test substance14C-salicylic acid14C-salicylic acid14C-salicylic acid
Temperature20 ± 2 °C20 ± 2 °C20 ± 2 °C
LightDarknessDarknessDarkness
Acclimation period21 days21 days21 days
Type of manureCattle (c), pig (p)Cattle (c), pig (p)Cattle (c), pig (p)
Manure sampling1 site: summer (c, p)1 site: winter (c, p)1 site: winter (c, p)
Dry matter content10 ± 1% (c), 5 ± 1% (p)10 ± 1% (c), 5 ± 1% (p)10 ± 1% (c), 5 ± 1% (p)
Amount of manure50 g fw50 g fw50 g fw or 300 g fw
Replicates per sampling6 (set in parallel)6 (set in parallel)6 (set in parallel)
Test duration85 days (c, p)91 days (c), 85 days (p)91 days (c), 85 days (p)
Sampling time pointsc: 3, 7, 14, 21, 35, 42, 49, 56, 63, 85
p: 3, 7, 14, 21, 35, 42, 49, 56, 63, 85
c: 3, 7, 14, 21, 29, 35, 42, 49, 56, 63, 70, 79, 91
p: 3, 7, 14, 21, 35, 42, 49, 56, 63, 85
c: 3, 7, 14, 21, 29, 35, 42, 49, 56, 63, 70, 79, 91
p: 3, 7, 14, 21, 35, 42, 49, 56, 63, 85
14CO2/14CH4 quantificationYes (each sampling point)Yes (each sampling point)Yes (each sampling point)
Residue characterizationYes (at the end)Yes (at the end)Yes (at the end)
Abiotic/sterile controlsNoNoNo
Microbial activity testingYes (at start and end)Yes (at start and end)Yes (at start and end)
Complete mass balanceYes (at the end)Yes (at the end)Yes (at the end)
Evaluation of kineticsYes (mineralization rate, DT50 MIN)Yes (mineralization rate, DT50 MIN)Yes (mineralization rate, DT50 MIN)