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Table 2 Summary of test conditions used for transformation studies to investigate the influence of test setup on mineralization rates of 14C-salicylic acid in cattle and pig manure (Test series 1)

From: Development of a test method for transformation of veterinary pharmaceuticals and biocides in anaerobic liquid manure

Parameter

Influence of test setup on mineralization rates (Test series 1)

Variations in test setup

(i) Flow-through vs. semi-static

(ii) Slow flow vs. fast flow

(iii) Low vs. high amount of manure

Details

Flow-through: 50–200 mL min−1

Semi-static: purged once/week

Slow flow: 50–200 mL/min

Fast flow: > 200 mL min−1

Low: 50 g manure fw

High: 300 g manure fw (slow flow-through)

Test substance

14C-salicylic acid

14C-salicylic acid

14C-salicylic acid

Temperature

20 ± 2 °C

20 ± 2 °C

20 ± 2 °C

Light

Darkness

Darkness

Darkness

Acclimation period

21 days

21 days

21 days

Type of manure

Cattle (c), pig (p)

Cattle (c), pig (p)

Cattle (c), pig (p)

Manure sampling

1 site: summer (c, p)

1 site: winter (c, p)

1 site: winter (c, p)

Dry matter content

10 ± 1% (c), 5 ± 1% (p)

10 ± 1% (c), 5 ± 1% (p)

10 ± 1% (c), 5 ± 1% (p)

Amount of manure

50 g fw

50 g fw

50 g fw or 300 g fw

Replicates per sampling

6 (set in parallel)

6 (set in parallel)

6 (set in parallel)

Test duration

85 days (c, p)

91 days (c), 85 days (p)

91 days (c), 85 days (p)

Sampling time points

c: 3, 7, 14, 21, 35, 42, 49, 56, 63, 85

p: 3, 7, 14, 21, 35, 42, 49, 56, 63, 85

c: 3, 7, 14, 21, 29, 35, 42, 49, 56, 63, 70, 79, 91

p: 3, 7, 14, 21, 35, 42, 49, 56, 63, 85

c: 3, 7, 14, 21, 29, 35, 42, 49, 56, 63, 70, 79, 91

p: 3, 7, 14, 21, 35, 42, 49, 56, 63, 85

14CO2/14CH4 quantification

Yes (each sampling point)

Yes (each sampling point)

Yes (each sampling point)

Residue characterization

Yes (at the end)

Yes (at the end)

Yes (at the end)

Abiotic/sterile controls

No

No

No

Microbial activity testing

Yes (at start and end)

Yes (at start and end)

Yes (at start and end)

Complete mass balance

Yes (at the end)

Yes (at the end)

Yes (at the end)

Evaluation of kinetics

Yes (mineralization rate, DT50 MIN)

Yes (mineralization rate, DT50 MIN)

Yes (mineralization rate, DT50 MIN)