Table 2 Validation parameters—retention time (Rt), relative retention time (Rel. Rt), linearity range, correlation coefficient obtained from calibration curve, instrumental limits of detection (LODS/N) and instrumental limits of quantification (LOQS/N)
Analytes | Rt (min) | Rel. Rt (min) | Linearity range (µg/L) | Calibration curve | R2 | LODS/N (µg/L) | LOQS/N (µg/L) |
|---|---|---|---|---|---|---|---|
AMP | 1.86 | 0.08 | 0.1–10 | Y = 19,828.7x − 554.71 | 0.9995 | 0.01 | 0.015 |
METH | 2.04 | 0.08 | 0.1–10 | Y = 40,079.6x − 265.875 | 0.9987 | 0.005 | 0.01 |
MC | 1.67 | 0.08 | 0.1–10 | Y = 36,872.3x − 1050.63 | 0.997 | 0.01 | 0.02 |
MDA | 1.93 | 0.07 | 0.1–10 | Y = 11,955.7x − 417.15 | 0.9992 | 0.02 | 0.025 |
MDMA | 2.04 | 0.08 | 0.1–10 | Y = 47,352.2x − 1614.27 | 0.9985 | 0.005 | 0.01 |
NK | 2.22 | 0.07 | 0.1–10 | Y = 36,217.3x − 663.94 | 0.999 | 0.01 | 0.02 |
KET | 2.29 | 0.07 | 0.1–10 | Y = 44,710.4x + 3013.45 | 0.9997 | 0.02 | 0.05 |
BE | 2.27 | 0.07 | 0.1–10 | Y = 23,465.0x − 597.23 | 0.9988 | 0.005 | 0.01 |
COD | 1.76 | 0.07 | 0.5–10 | Y = 2682.0x − 131.27 | 0.9978 | 0.01 | 0.05 |
COC | 2.74 | 0.07 | 0.1–10 | Y = 21,003.5x − 10.74 | 0.997 | 0.025 | 0.02 |
MET | 3.99 | 0.08 | 0.1–10 | Y = 27,369.3x − 4660.57 | 0.9988 | 0.01 | 0.02 |
HR | 2.64 | 0.07 | 0.1–10 | Y = 803.6x − 55.84 | 0.995 | 0.02 | 0.05 |