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Table 1 Biotests used to detect effect potentials in environmental samples (effluent, surface water, and sediment) in the project SchussenAktivplus

From: Freshwater ecosystems profit from activated carbon-based wastewater treatment across various levels of biological organisation in a short timeframe

Testing for

Biotest used

According to

Genotoxicity

Ames-fluctuation test

ISO 11350 guideline, described in detail by Giebner et al. [37]

SOS Chromotest

White et al. [38]

Dioxin-like toxicity

Reporter gene assay with H4IIE-luc-cells

Garrison et al. [39], described in detail by Maier et al. [32]

Yeast Dioxin Screen

Stalter et al. [40]

(Anti-) Oestrogenicity

Y(A)ES

Stalter et al. [40], described in detail by Giebner et al. [37]

E-Screen

Lange et al. [41]

Reporter gene assay with HeLA cells

US EPA [42], described in detail by Henneberg et al. [43]

(Anti-) Androgenicity

Y(A)AS

Stalter et al. [40],

described in detail by Giebner et al. [37]

Reporter gene assay with MDA-kb2-cells

Wilson et al. [44]

Phytotoxicity

Lemna minor growth inhibition test

OECD 221 [45]

Embryo- and developmental toxicity

Fish embryo acute toxicity test (FET) with Danio rerio

OECD 236 [46], described in detail by Thellmann et al. [47]

Effects on reproduction

Sediment and water toxicity test with Lumbriculus variegatus

OECD 225 [48]

Potamopyrgus antipodarum reproduction test

OECD 242 [49], described in detail by Giebner et al. [37] and Henneberg et al. [43]