Table 2 PCR amplification of OsPCR gene and its interference fragment sequence primer information
From: A novel family of PLAC8 motif-containing/PCR genes mediates Cd tolerance and Cd accumulation in rice
Primer | Primer sequence | Tm | Sequence length (bp) |
|---|---|---|---|
OsPCR1-F OsPCR1-R | 5′-GGTACCATGTACTCGAAACCGGAGG-3′ 5′-GTCGACGCGTGTCATCCCGGGGA-3′ | 55 | 489 |
OsPCR3-F OsPCR3-R | 5′-GGTACCATGTATCCCTCCGCCCCT-3′ 5′-GTCGACCCTCATCATGCCGACCGG-3′ | 58 | 546 |
OsPCR1RNAi-F OsPCR1RNAi-R | 5′- CACCATGTACTCGAAACCGGAGGA-3′ 5′-CACAGGCACGTCACGCAACAGTT-3′ | 59 | 140 |
OsPCR3RNAi-F OsPCR3RNAi-R | 5′-CACCATGTATCCCTCCGCCCCTCC-3′ 5′-CGTCCATGCAGTGGAAAAGGCCGGTG-3′ | 64 | 170 |