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Table 2 PCR amplification of OsPCR gene and its interference fragment sequence primer information

From: A novel family of PLAC8 motif-containing/PCR genes mediates Cd tolerance and Cd accumulation in rice

Primer Primer sequence Tm Sequence length (bp)
OsPCR1-F
OsPCR1-R
5′-GGTACCATGTACTCGAAACCGGAGG-3′
5′-GTCGACGCGTGTCATCCCGGGGA-3′
55 489
OsPCR3-F
OsPCR3-R
5′-GGTACCATGTATCCCTCCGCCCCT-3′
5′-GTCGACCCTCATCATGCCGACCGG-3′
58 546
OsPCR1RNAi-F
OsPCR1RNAi-R
5′- CACCATGTACTCGAAACCGGAGGA-3′
5′-CACAGGCACGTCACGCAACAGTT-3′
59 140
OsPCR3RNAi-F
OsPCR3RNAi-R
5′-CACCATGTATCCCTCCGCCCCTCC-3′
5′-CGTCCATGCAGTGGAAAAGGCCGGTG-3′
64 170