Table 1 PCR primers used for analysis of gene expression
From: Liver toxicity assessments in rats following sub-chronic oral exposure to copper nanoparticles
Target | Sequences of primers (5′ to 3′) | Base number |
|---|---|---|
CYP450 1A2 F | GGTGGAATCGGTGGCTAAT | 19 |
CYP450 1A2 R | AGTCCTTGCTGCTCTTCACG | 20 |
CYP450 2C11 F | AATCCGCAGTCTGAGTTTACCC | 22 |
CYP450 2C11 R | GGTTTCTGCCAATTACACGTTCT | 23 |
CYP450 2D6 F | AGCTTCAACACCGCTATGGT | 20 |
CYP450 2D6 R | CAGCAGTGTCCTCTCCATGA | 20 |
CYP450 2E1 F | CCTTTCCCTCTTCCCATCC | 19 |
CYP450 2E1 R | AACCTCCGCACATCCTTCC | 19 |
CYP450 3A1 F | TGCCATCACGGACACAGA | 18 |
CYP450 3A1 R | ATCTCTTCCACTCCTCATCCTTAG | 24 |
PXR R | TGATGACGCCCTTGAACATG | 20 |
CAR F | CCACGGGCTATCATTTCCAT | 20 |
CAR R | CCCAGCAAACGGACAGATG | 19 |
AHR F | TGGACAAACTCTCCGTTCTAAGG | 23 |
AHR R | GATTTTAATGCAACATCAAAGAAGCT | 26 |
GAPDH F | GATGGTGAAGGTCGGTGTG | 19 |
GAPDH R | ATGAAGGGGTCGTTGATGG | 19 |