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Table 4 Inhibition values (%) based on M. aquaticum growth parameters and physico–chemical properties of natural sediments

From: A contribution towards improving the applicability of the Myriophyllum aquaticum sediment contact test

Parameter

Natural sediments

 

T6

T7

T8

T9

T10

T11

T12

T13

T14

J15

K16

L17

L18

L19

Inhibition (I%)

RGR

31.7*

52.1*

45.4*

17.6

4.8

34.9*

62.9*

37.8

53.7*

20.8*

17.3*

-3.8

5.7

4.4

wFW

24.4

34.8*

33.6*

19.7

20.2

38.2*

39.1*

21.7

37.7

11.7

5.4

-14

1.2

9.8

sFW

75.1*

71.9*

69*

50.7*

49.6*

79.5*

76.3*

66.1*

75*

32.4*

17.8

21.13

30.26

37.85*

sL

45.2*

35.3*

37.3*

17.5

15.4

54.6*

48.8*

28.9

36.7

-5.3

-10.8

23.1

45.9*

49.9*

rFW

-321*

-105.6

-141.8*

-205.1*

-182.9*

-121.6*

27.2

-113.1*

-10.2

28.2

55.9

-281.8*

-232.6*

-241.2*

rL

-201.2*

-224.3*

-276.5*

-127*

-124.3

-57.1

-114.7

-416.2*

-120.4

-33.3

-7.5

-100.5*

-128.6*

-107.9*

Sediment toxicity**

T

T

T

NT

NT

T

T

T

T

T

NT

NT

NT

NT

 

Physico-chemical analysis

 

Sand, %

74

79

83

74

79

83

74

79

83

69.4

73.2

45.3

59.6

64.3

Thresholds

Silt and clay, %

13

8

15

13

8

15

13

8

15

28.4

23.7

28.3

25.4

21.4

TECa

PECa

Org. matter, %

3.8

7.69

6.94

8.1

8.54

2.75

2.07

7.56

8.18

9.16

10.1

26.4

15

14.4

Phenanthrene, µg/kg

5.53

2.27

6.64

8.28

2.03

10.7

17.6

27.3

95.7

16.08

80.5

835

14.7

29.6

204

1170

Fluoranthene , µg/kg

1.52

3.23

2.75

2.25

1.17

5.87

6.82

12.3

86.6

7.8

6.93

538

12.3

24.9

423

2230

Heptachlor, µg/kg

0

1.68

0

0

0

0

2.03

0

13.2

0

0

0

0

0

0.7b

68b

Hep. epoxide, µg/kg

22.6

55.3

2.03

0

3.18

5.29

0

0

0

0

0

0

0

0

2.47

16

Aldrin, µg/kg

1.39

0

0

0

0

0

0

0

0

0.32

0

0

0

0

0.06b

6b

Endrin, µg/kg

7.27

0

0

0

1.91

0

0

0

0

0

0

0

0

0

2.22

207

4,4’-DDE, µg/kg

1.07

0

1.55

0

1.04

0.8

0

1.02

0

0

1.17

0

0

0

3.16

31.3

4,4’-DDD, µg/kg

0

0

0

0

0.75

0

0

0

0

0

0

0

0

0

4.88

28

Sum PAH, µg/kg

1.52

2.23

2.75

2.25

1.17

7.14

31.4

35.9

340

n.m.

n.m.

n.m.

n.m.

n.m.

1610

22800

PCB sum, µg/kg

0

0

0

0

83.3

91.1

0

0

0

0

0

0

0

0

59.8

676

Mineral oils, mg/kg

1430

1480

1070

940

1480

1260

1250

1620

1490

0

3790

5619

0

57.2

50b

3000b

Cd, mg/kg

0.36

1.03

2.36

0.76

1.82

3.34

0

0.22

0.44

0

2.25

0

0.08

0.12

0.99

4.98

Pb, mg/kg

37.2

46.2

64.9

7.3

11.2

13.8

31.8

17.3

20.8

25.3

0

104.3

15.4

16.4

35.8

128

Hg, mg/kg

0.04

0.21

2.47

0.05

0.14

0.15

0.07

0.21

0.13

0.1

0

2.09

0.18

0.17

0.18

1.06

As, mg/kg

25.8

17.4

35.5

36.6

36.6

44.3

6.73

4.11

4.59

31.7

0

217

40.5

31.3

9.79

33

Ni, mg/kg

nm

nm

nm

nm

nm

nm

nm

nm

nm

nm

nm

147.7

8.24

8.91

22.7

48.6

Zn, mg/kg

nm

nm

nm

nm

nm

nm

nm

nm

nm

nm

nm

698.4

20.55

22.61

121

459

Cr, mg/kg

nm

nm

nm

nm

nm

nm

nm

nm

nm

nm

nm

1178

13.03

15.18

43.4

111

Cu, mg/kg

nm

nm

nm

nm

nm

nm

nm

nm

nm

nm

nm

347

16.55

15.49

31.6

149

  1. RGR, relative growth rate; wFW, whorl fresh weight; sFW at d10-shoot fresh weight; sL, shoot length; rFW, root fresh weight; rL, root length; T, toxic; NT, not toxic; nm, not measured, 0, below the method detection limit (MDL) or the practical quantification limit (PQL)
  2. * Significantly different from the corresponding test control [one-way ANOVA followed by t test, p ≤ 0.05)]
  3. **Sediment toxicity based on the M. aquaticum test (I% RGR) according to Höss et al [13]
  4. a[MacDonald et al. 26], b [Official Gazette of Republic of Serbia, 50/12 Službeni Glasnik Republike Srbije 35]. Surpassed TEC or Serbian TV are marked with italics letters, while values above PEC and Serbian MAC are marked with underlined cell borders