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Table 3 Annual average activities and standard deviations of estrogenic, anti-estrogenic and dioxin-like activities and EC50 in the microtox assay referring to the REF for baseline toxicity of water samples

From: Detection of chemically induced ecotoxicological effects in rivers of the Nidda catchment (Hessen, Germany) and development of an ecotoxicological, Water Framework Directive–compliant assessment system

River

Water sample

YES [EEQ ng/L]

YAES [OHT-EQ mg/L]

YDS [β-NF-EQ µg/L]

Microtox [EC50 REF]

Nidda

 N1

0.111 ± 0.069

1.24 ± 0.802

< 0.321

750 ± 0

 N2

0.208 ± 0.241

1.27 ± 0.802

0.602 ± 0.364

644 ± 213

 N3

0.163 ± 0.155

1.34 ± 0.818

0.467 ± 0.410

750 ± 0

 N4

0.185 ± 0.198

1.55 ± 0.994

0.626 ± 0.229

511 ± 278

Usa

 U1

< 0.073

< 0.400

0.418 ± 0.301

750 ± 0

 U2

< 0.073

< 0.400

0.419 ± 0.311

661 ± 179

 U3

< 0.073

< 0.400

0.357 ± 0.511

593 ± 315

 U4

0.131 ± 0.075

8.00 ± 7.17

0.533 ± 0.358

530 ± 254

Horloff

 H1

< 0.073

< 0.400

< 0.321

370 ± 335

 H2

< 0.073

< 0.400

< 0.321

598 ± 263

 H3

0.157 ± 0.031

< 0.400

0.453 ± 0.078

428 ± 301

 H4

< 0.073

< 0.400

0.453 ± 0.080

533 ± 194

  1. For non-toxic water samples in the microtox assay the EC50 threshold value is 750 REF