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Table 2 Overview of the reference and sampling sites at the rivers Nidda, Usa, and Horloff

From: Detection of chemically induced ecotoxicological effects in rivers of the Nidda catchment (Hessen, Germany) and development of an ecotoxicological, Water Framework Directive–compliant assessment system

Sampling site

Latitude [N]

Longitude [E]

Characteristics

Nidda

 N1

50°27′11″

9°02′53″

Reference site without any upstream dischargers/effluents; downstream of the Nidda river dam, surrounded by intensive agriculture

 N2

50°19′41″

8°52′32″

Downstream of two WWTPs (35,000 and 7500 PE) and a paper mill, surrounded by intensive agriculture

 N3

50°19′08″

8°51′28″

Downstream of the mouth of the tributary Horloff, within the city Nieder-Flortstadt

 N4

50°16′42″

8°47′08″

Downstream of two WTTPs (30,000 and 12,000 PE), within restored area from 2001, surrounded by intensive agriculture

Usa

 U1

50°19′01″

8°31′26″

Reference site without any upstream dischargers/effluents, surrounded by intensive agriculture

 U2

50°22′48″

8°42′45″

Downstream of a WWTP (49,000 PE), surrounded by garden plots

 U3

50°21′32″

8°44′39″

Within restored area from 2010, within the city Bad Nauheim

 U4

50°20′09″

8°46′16″

Downstream of a WWTP (43,800 PE) and discharges from public baths, surrounded by intensive agriculture and garden plots

Horloff

 H1

50°31′12″

9°02′36″

Reference site without any upstream dischargers/effluents, surrounded by forests and some cottages

 H2

50°30′52″

8°57′00″

Downstream of four small-sized WWTPs (< 1000 PE), surrounded by intensive agriculture

 H3

50°24′41″

8°54′02″

Downstream of one WWTP (78,000 PE), surrounded by intensive agriculture

 H4

50°23′57″

8°53′56″

Within restored area from 2006/2007, surrounded by intensive agriculture