Table 1 Sequences and efficiencies of primers used in quantitative real-time PCR experiments
Gene | Accession number | Forward primer | Reverse primer | Primers efficiency | Coefficient of determination | Annealing temperature (°C) |
|---|---|---|---|---|---|---|
β-actin | NM_131031 | TGCTGTTTTCCCCTCCATTG | TTCTGTCCCATGCCAACCA | 0.97 | 0.99 | 60 |
cyp1c1 a | NM_001020610 | TGGAGGCTGAGTTTGGACTGAAGA | GAGGAAGAAGAGGATGACGAAGGATG | 0.982 | 0.99 | 60 |
cyp3c4 | NM_001077548 | TTAACAGAAGGGTAAGCTCTCCAAA | GCCAGCCAGTCCTGTCGT | 0.942 | 0.98 | 60 |
cyp3A65 | NM_001037438.1 | GTTCTTGGTGGCGCTGCT | AAGTACCTATGAGGCCAGGATCC | 1 | 0.99 | 60 |
gstp1 | NM_131734.3 | ATGAAGGGCGACTTGAAAGC | CATGGCGTTGGACTGAAACA | 0.995 | 0.99 | 60 |